pShuttle-IRES-hrGFP-2载体
- 品牌：ZYbscience
- 型号：泽叶生物
- 产地：中国/美国
- 供应商：上海泽叶生物科技有限公司
- 供应商报价：¥2000
- 标签：pShuttle-IRES-hrGFP-2载体、pShuttle-IRES-hrGFP-2载体价格、pShuttle-IRES-hrGFP-2载体厂家、pShuttle-IRES-hrGFP-2、、上海泽叶生物科技有限公司
产品库
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pShuttle-IRES-hrGFP-2 |
| 质粒类型: | 腺病毒载体；双顺反子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8.9 kb |
| 5' 测序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 测序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 备注: | 载体内包含有IRES序列和hrGFP序列。 |
| 产品目录号: | ZY240082 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
