pLVX-DD-ZsGreen1 Reporter载体

LOCUS       pLVX-DD-ZsGreen1_Reporter   8454 bp    DNA   circular             6-NOV-2009COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT     Cat. No. 631751,631752FEATURES             Location/Qualifiers     LTR             1..635                     /label=5'\LTR     misc_feature    685..822                     /label=packaging\signal     misc_feature    4422..5013                     /label=WPRE     rep_origin      complement(6322..6995)                     /label=pUC\origin     CDS             complement(7140..8136)                     /label=Amp(R)     misc_binding    1303..1536                     /label=RRE     misc_binding    636..653                     /label=PBS     misc_feature    2028..2151                     /label=cPPT/CTS     LTR             5216..5852                     /label=3'\LTR     misc_feature    3280..3788                     /label=PGK\Promoter     misc_feature    3809..4408                     /label=Puro(R)     misc_feature    2195..2234                     /label=MCS     CDS             2247..2570                     /label=DD     CDS             2577..3269                     /label=ZsGreen1BASE COUNT     2089 a      2198 c      2253 g      1914 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg      2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag      2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg      2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag      2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga      2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca      2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg      2581 cccagtccaa gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg      2641 tggacggcca caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc      2701 aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt      2761 ctgccgcctt catgtacggc aaccgcgtgt tcaccgagta tccccaggac atcgtcgact      2821 acttcaagaa ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg      2881 gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc      2941 acgagtccaa gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga      3001 ccgacaactg ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga      3061 agggcgacgt gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg      3121 acaccgtgta caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc      3181 acaagctgac ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc      3241 acgccatcgc ctccggctcc gccttgccct agtctagata attctaccgg gtaggggagg      3301 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct      3361 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc      3421 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc      3481 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc      3541 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc      3601 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg      3661 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg      3721 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg      3781 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc      3841 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc      3901 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga      3961 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc      4021 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat      4081 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg      4141 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc      4201 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc      4261 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta      4321 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg      4381 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca      4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat      4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct      4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac      4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca      4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca      4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg      4801 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga      4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt      4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga      4981 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga      5041 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct      5101 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc      5161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga      5221 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca      5281 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac      5341 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa      5401 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag      5461 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct      5521 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg      5581 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg      5641 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct      5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag      5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac      5821 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt      5881 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt      5941 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga      6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc      6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc      6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc      6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt      6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca      6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa      6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat      6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc      6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc      6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt      6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac      6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg      6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca      6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc      6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa      6901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa      6961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac      7021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta      7081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt      7141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata      7201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc      7261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac      7321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag      7381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac      7441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc      7501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg      7561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc      7621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct      7681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc      7741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc      7801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc      7861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc      7921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca      7981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt      8041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt      8101 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt      8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc      8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt      8281 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc      8341 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt      8401 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt