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载体pMIR-Reporter

产品信息
  • pMIR-Reporter
    产品信息
    产品货号 产品名称 产品规格 优惠价
    HZ0471 pMIR-Reporter

    20μl

    ¥请询价
    使用说明
    沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
    基本信息
    启动子: CMV,SP6,T7,Lac
    复制子: pUC ori,SV40 ori
    终止子: SV40 poly(A) signal
    质粒分类: 哺乳细胞,信号通路报告载体
    质粒大小: 6470bp
    原核抗性: Amp
    筛选标记: Puro
    克隆菌株: DH5α
    培养条件: 37℃,有氧 LB
    表达宿主: 哺乳细胞
    诱导方式: 无须诱导,瞬时表达
    5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
    3'测序引物: M13F:TGTAAAACGACGGCCAGT
    质粒简介
         The pMIR-REPORT™ miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.
    质粒图谱
    温馨提示:不可用于临床ZL。
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