pLVX-Hom-Mem1载体

注：When cloning your gene of interest into the pLVX-Hom-Mem1 vector, make certain that the N-myr-signal is located on the N-terminus of the DmrB fusion protein. The N‐myr‐signal is NOT functional if localized in the middle or at the C‐terminus of a protein of interest. Your gene must either be cloned into the 3’MCS, or if cloned into the 5’ MCS, which is located upstream of the N‐myr signal, you must include a separate N‐myr signal sequence on the forward primer used to amplify your gene of interest.

LOCUS       pLVX-Hom-Mem1          8841 bp ds-DNA    circular SYN 30-JUL-2013COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comKEYWORDS    pLVX-Hom-Mem1 VectorSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 8841)  AUTHORS   Clontech Laboratories Inc.FEATURES             Location/Qualifiers     source          1..8841                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     LTR             1..634                     /label="5' LTR"                          misc_feature    681..806                     /label="Packaging signal"     misc_feature    1303..1536                     /label="RRE"     misc_feature    2028..2144                     /label="cPPT/CTS"     enhancer        2201..2504                     /label="CMV enhancer"     promoter        2505..2708                     /label="CMV promoter"     misc_feature    2803..2820                     /label="5' MCS"     misc_feature    2821..2862                     /label="N-myr signal"     CDS             2869..3189                     /label="DmrB"     CDS             3196..3516                     /label="DmrB"     misc_feature    3518..3531                     /label="3' MCS"     misc_feature    3544..4117                     /label="IRES"     CDS             4150..4749                     /label="PuroR"     misc_feature    4763..5351                     /label="WPRE"     LTR             5559..6192                     /label="3' LTR"     rep_origin      complement(6723..7308)                     /direction=LEFT                     /label="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(7479..8339)                     /label="AmpR"     promoter        complement(8340..8444)                     /label="AmpR promoter"ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt     2821 atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg cgtccaagtc     2881 gaaaccatta gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc     2941 cattatacag gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa     3001 cctttcaaat tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct     3061 caaatgtccg tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct     3121 accggacatc ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg     3181 aagctcgaga ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc     3241 cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa     3301 gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg     3361 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact     3421 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc     3481 actctcgtct tcgatgtgga gcttctaaaa ctggaaagcg gccgcggatc ctacgtatga     3541 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg     3601 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc     3661 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa     3721 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag     3781 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg     3841 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg     3901 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa     3961 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg     4021 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca     4081 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga     4141 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc     4201 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc     4261 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc     4321 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc     4381 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag     4441 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg     4501 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag     4561 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc     4621 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc     4681 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc     4741 ggtgcctaga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac     4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt     4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt     4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt     4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg     5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg     5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct     5161 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt     5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc     5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc     5341 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca     5401 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag     5461 gaggtgggtt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc     5521 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca     5581 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta     5641 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa     5701 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt     5761 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag     5821 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa     5881 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg     5941 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt     6001 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac     6061 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg     6121 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga     6181 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga     6241 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc     6301 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca     6361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg     6421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg     6481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc     6541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     6601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     6661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     6721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     6781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     6841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     6901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     6961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     7021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     7081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     7141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     7201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     7261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     7321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     7381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     7441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     7501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     7561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     7621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     7681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     7741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     7801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     7861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     7921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     7981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     8041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     8101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     8161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     8221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     8281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     8341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     8401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     8461 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta     8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag     8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca     8641 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct     8701 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt     8761 gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat     8821 atgtactaca aacttagtag t//