Cynomolgus TIGIT Protein

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA against immobilized Human CD155, Fc Tag (Cat# CD5-H5251, 1 μg/well). The linear range is 0.7-20 μg/mL.

产品背景:
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

产品描述:
Cynomolgus TIGIT, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 15.2 kDa. The predicted N-terminus is Met 89. The reducing (R) protein migrates as 20-23 kDa in SDS-PAGE.