终末分化标志物PCR芯片Terminal Differentiation Markers PCR Array

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Terminal Differentiation Markers PCR Array

终末分化标志物PCR芯片
 

Product	Species	Technology	Cat. No.
Terminal Differentiation Markers PCR Array	Human	Gene Expression	PAHS-048Z
Terminal Differentiation Markers PCR Array	Mouse	Gene Expression	PAMM-048Z
Terminal Differentiation Markers PCR Array	Rat	Gene Expression	PARN-048Z

    Terminal Differentiation Markers RT² Profiler™ PCR Array profiles the expression of 84 key genes important for the identification of specific cell types. The complex and time-consuming process of successfully differentiating embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) into a specific cell type requires verification by testing positive cellular markers before proceeding with experiments. However, further examination of other cell type markers serves as an equally important negative control confirming the specificity of the differentiation program. For example, iPSCs differentiated into arterial endothelial cells should express arterial markers but not venous markers. Or, the expression of cardiomyocyte markers confirms successful differentiation into that cell type, and the absence of markers for cells from other major organs (such as lung, pancreas and brain) insures the lack of other non-relevant cell types. This array provides the complete answer for cellular identification after differentiation. It includes one to three specific gene expression markers from 13 major organ or cellular types, with up to seven subgroups of more specific types of cells. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes for cellular identification with this array.

    终末分化标志物PCR芯片可用于研究84个特定细胞类型鉴定关键基因的表达。使胚胎干细胞或诱导性多能干细胞(iPSCs)分化成特定的细胞类型是一个非常繁琐和费时的过程，需要在实验进行前检测多种阳性细胞标志物来进行验证。而且，对其它细胞类型标志物的进一步检测作为阴性对照对分化过程也同样重要。例如，iPSCs分化成血管内皮细胞应该表达动脉标志物而不是静脉标志物，又或者心肌细胞标志物的表达说明细胞成功分化成了心肌细胞，而无其它主要器官（如肺，和脑）标志物的表达则确定了分化细胞中没有其它非相关细胞类型的存在。该芯片为分化后细胞类型的鉴定提供了全面的解决方案，它包含了13种主要器官或细胞类型以及7种相对特殊细胞类型的各1-3个基因表达标志物。通过实时定量PCR，研究者可以简单可靠地同时分析细胞类型鉴定相关基因的表达。
Adipocyte:ADIPOQ, FABP4, PPARG.
Chondrocyte:ACAN, COL10A1, COMP.
Endothelium:
General:CDH5, KDR, PECAM1. 
Arterial:DLL4, EFNB2, NRP1. 
Lymphatic:LYVE1, PROX1. 
Venous:NR2F2, NRP2.
Epithelium:
Keratinocyte:KRT1, KRT10, KRT14.
Melanocyte:PMEL, TYR, TYRP1.
Bone:
Osteoblast:BGLAP, COL2A1, IBSP.
Osteoclast:CALCR, CTSK.
Liver:
Cholangiocyte:ITGB4, KRT19.
Hepatocyte:ALB, G6PC, TAT.
Bone Marrow:
Early B cell Development:CD79A.
Early T Cell Development:CD3E, PTCRA.
Macrophage:CCR5, CXCR4, EMR1.
Monocyte:ITGAM.
Nervous System:
Glial Cell:GALC, GFAP.
Mature Neuron:MAP2, NEFH, TUBB3.
Cholinergic Neuron:CHAT.
Dopaminergic Neuron:TH.
GABA Neuron:GAD1, SLC32A1.
Glutamatergic Neuron:SLC17A6, SLC17A7.
Motor Neuron:ISL1.
Oligodendrocyte:MBP.
Retina:
Ganglion Cell:POU4F2.
Muller Cell:RLBP1.
Photoreceptor Cell:PDE6B, RCVRN.
Kidney (Nephron):
Podocyte:NPHS2.
Proximal Tubule Cell:AQP1, CYP27B1, MIOX.
Collecting Duct Cell:AQP2.
Distal Tubule CellUMOD.
Lung:SFTPB, SFTPC, SFTPD.
Muscle:
Cardiomyocyte:MYH6, MYH7, NPPA.
Skeletal Muscle Cell:CAV3, MYH1, MYOD1
Smooth Muscle Cell:MYH11, SMTN, TAGLN.
Pancreas:
Alpha Cell:GCG, MAFB, POU3F4.
Beta Cell:INS, MAFA, SLC2A2.
Delta Cell:SST.
Epsilon Cell:GHRL.
PP Cell:PPY.
Exocrine Cell:CPA1.