Human Adenomatous polyposis coli protein(APC) ELISA kit标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.770 2.713 2.742 2.628
1500 1.574 1.555 1.565 1.451
750 0.774 0.762 0.768 0.654
375 0.499 0.521 0.510 0.396
187.5 0.292 0.307 0.300 0.186
94 0.223 0.231 0.227 0.113
47 0.189 0.184 0.187 0.073
0 0.115 0.113 0.114
Human Adenomatous polyposis coli protein(APC) ELISA kit精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human Adenomatous polyposis coli protein(APC) ELISA kit线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 89
Range % 85-92
1:2 Average % 101
Range % 97-104
1:4 Average % 93
Range % 90-95
1:8 Average % 90
Range % 86-94
Human Adenomatous polyposis coli protein(APC) ELISA kit回收率:
The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 86-94
EDTA plasma (n=4) 87 84-92
