Product Name:Human Adenomatous polyposis coli protein, APC ELISA KIT
Packing:96T
Catalog No.:ELI-11715h
Gene Name:APC
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Adenomatous polyposis coli protein(APC
Alternative Name:APC,Adenomatous polyposis coli protein(APC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Adenomatous polyposis coli protein, APC ELISA KIT allows for the in vitro quantitative determination of Adenomatous polyposis coli protein(APC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Adenomatous polyposis coli protein, APC ELISA KIT has been pre-coated with an Adenomatous polyposis coli protein(APC antibody specific to Adenomatous polyposis coli protein(APC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Adenomatous polyposis coli protein(APC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Adenomatous polyposis coli protein(APC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Adenomatous polyposis coli protein(APC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
