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Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody

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  • Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody详细信息:
    Product Overview

     

    Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody was screened on human stem cells using immunocytochemistry (ICC) and flow cytometry (FC), and selected as the best application-tested secondary antibody, among those screened, for stem cell research applications. This polyclonal goat anti-rat IgG + IgM antibody reacts with rat IgG, IgM, and the light chains of other rat immunoglobulins. Cross reactivity against non-immunoglobulin serum proteins has not been detected. The Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody has been solid-phase absorbed and tested by ELISA to ensure minimal cross-reactivity with human, bovine, and horse immunoglobulins. However, cross-reaction with immunoglobulins from other species has not been tested.

    Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM AntibodyProduct Specifications

    Reactivity    Rat
    Clone    Polyclonal
    Isotype    Goat IgG
    Lot Number    1280
    Quantity / Size    100 μg; 0.2 ml (0.5 mg/ml)
    Formulation    Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide.
    Preparation    This antibody was purified by affinity chromatography and conjugated with DyLight™ 488 under optimal conditions.
    Fluorophore    DyLight™ 488 is spectrally similar to Alexa Fluor® 488, fluorescein, and FITC. The excitation maximum is 493 nm. The emission maximum is 518 nm.
    Storage    Store undiluted at 4°C and protected from light. Do not freeze.
    Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM AntibodyStability    Stable for 6 months from date of receipt when stored as directed
    Quality Control    Tested by immunocytochemistry and flow cytometry
    Recommended Usage    Dilute 1:100 for immunocytochemistry and 1:20 for flow cytometry. For general protocols for performing immunocytochemistry and flow cytometry directly on stem cells, see Technical Documents on the Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody product page at www.stemgent.com/cat09-0035.
    Notice to Purchaser    Purchaser represents and warrants that it will use the Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody purely for research purposes. This product is not intended for human or veterinary applications, or diagnostic use or resale. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of this product.
    Related Products    
    Affinity Purified anti-Human SSEA-3 (Cat.No.09-0014)
    Stemgent® Purified Rat anti-Mouse/Human CD44 Antibody (Cat.No.09-0027)
    Additional References    DyLight™ is a trademark of Thermo Fisher Scientific. 
    Alexa Fluor® is a registered trademark of Invitrogen Corporation.
    Stemgent® DyLight™ 488 Goat anti-Rat IgG + IgM Antibody
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