Stemgent® DyLight™ 549 Mouse anti-Human Tra-1-81 Antibody详细信息：
Product Overview
Stemgent® DyLight™ 549 Mouse anti-Human TRA-1-81 Antibody was screened on human embryonic stem (ES) cells using immunocytochemistry (ICC). The TRA-1-81 monoclonal antibody reacts with a pluripotent stem cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Stemgent® DyLight™ 549 Mouse anti-Human Tra-1-81 AntibodyReferences
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell 131, 861-872.
Yu J., Vodyanik M.A., Smuga-Otto K., Antosiewicz-Bourget J., Frane J.L., Tian S., Nie J., Jonsdottir G.A., Ruotti V., Stewart R., Slukvin I.I., Thomson J.A. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920.
Schopperle W.M., DeWolf W.C. (2007). The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25, 723-730.
Chin A.C., Fong W.J., Goh L.T., Philp R., Oh S.K., Choo A.B. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 130, 320-328.
Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P. (1984). Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3, 347.
Lowry, W.E., Richter, L., Yachechko, R., Pyle, A.D., Tchieu, J., Sridharan, R., Clark, A.T., Plath, K. (2008) Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A 105: 2883-8.
Product Specifications
Stemgent® DyLight™ 549 Mouse anti-Human Tra-1-81 AntibodyImmunogen Human embryonal carcinoma cell line 2102Ep
Reactivity Human
Clone TRA-1-81
Isotype Mouse IgM,κ
Formulation Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with Dylight™ 549 under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage Store undiluted at 4oC. Do not freeze. Light sensitive.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunocytochemistry to ensure product quality.
Recommended Usage Use antibody at 1:100 in ICC. For general protocols for immunocytochemistry on stem cells, see the Technical Documents on the Stemgent® Dylight™ 549 Mouse anti-Human Tra-1-81 product page.
Related Products
Stemgent® Affinity Purified anti-Human TRA-1-81 Antibody (Cat.No.09-0011)
Stemgent® Phycoerythrin (PE) anti-Human TRA-1-81 Antibody (Cat.No.09-0012)
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody (Cat.No.09-0069)
Trademark DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
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