Stemgent® Purified Mouse anti-Mouse/Human βIII Tubulin Antibody详细信息：
Product Overview
The Stemgent® Purified Mouse anti-Mouse/Human βIII-Tubulin Antibody was screened on neural cells derived from mouse and human ES cells via directed differentiation and selected as the best application-tested antibody for βIII-tubulin. This antibody was generated against the peptide (ESESQGPK) derived from the C-terminus of the human βIII-tubulin protein. Tubulin is the major constituent of microtubules, existing as a heterodimer of α and β subunits. βIII-tubulin is regarded as a specific probe for the cells of neuronal origin as well as for tumors originating from these cells. The expression of βIII tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium1-8.
Stemgent® Purified Mouse anti-Mouse/Human βIII Tubulin AntibodyProduct Specifications
Immunogen Peptide (C) 441-448 coupled to maleimide-activated KLH via cysteine added to the N-terminus of the neuron-specific peptide
Reactivity Human, Mouse
Clone TU-20
Isotype Mouse IgG1
Formulation Phosphate-buffered saline and 15mM sodium azide, pH 7.4
Preparation This antibody was purified from ascites by precipitation methods.
Storage Store undiluted at 4°C. For long-term storage aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Stemgent® Purified Mouse anti-Mouse/Human βIII Tubulin AntibodyStability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunocytochemistry.
Recommended Usage Dilute 1:100 for immunocytochemistry. Reported applications include Western blot, flow cytometry and IHC staining of tissue sections. For general protocol for performing immunocytochemistry on ES cells, see Technical Documents.
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