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GenMute™ siRNA Transfection Reagent for Primary Keratinocytes

产品信息

  • Description:
    GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.

    Size & content:
    - GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
    - GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

    Refer to the following optimal transfection conditions for maximal silencing on primary keratinocytes. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

    Summary of Optimal Transfection Conditions:
    Cell culture medium
    Confluency of cell on the day of transfection
    Optimal siRNA concentration
    Diluent for siRNA and GenMute    ™ Reagent
    Incubation Time to Form GenMute™/siRNA Complex
    Maximal Silencing Efficiency 

    Transfection Results:
    Reporter Gene
    Silencing Efficiency (% )


    DMEM with 4.5 g/L glucose, 10% FBS
    ~70%
    40 nM
    1xGenMute™ Transfection Buffer
    ~15 minutes at RT
    48 hours


    Renilla Luciferase
    90%


    Storage:
    Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

    Data Sheet & Protocol.pdf
    温馨提示：不可用于临床ZL。
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