内皮糖蛋白(ENG)重组蛋白

内皮糖蛋白(ENG)重组蛋白 Recombinant Endoglin (ENG)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species    Mus musculus (Mouse)
Product No.    RPA980Mu02
Source    Prokaryotic expression
Host    E.coli
Purity    > 95%
UOM    50ug
Predicted Molecular Mass    13.9kDa
Predicted isoelectric point    n/a
Applications    SDS-PAGE; WB; ELISA; IP.
Endotoxin Level    <1.0EU per 1µg (determined by the LAL method)
内皮糖蛋白(ENG)重组蛋白 Subcellular Location    n/a
Residues    Thr336~Phe450 (Accession # Q63961) with N-terminal His-Tag
Formulation    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-TTPAP VVTTPPKDTC SPVLLMSLIQ PKCGNQVMTL ALNKKHVQTL QCTITGLTFW DSSCQAEDTD DHLVLSSAYS SCGMKVTAHV VSNEVIISFP SGSPPLRKKV QCIDMDSLSF
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
内皮糖蛋白(ENG)重组蛋白 Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
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