皮质醇结合球蛋白(CBG)重组蛋白

皮质醇结合球蛋白(CBG)重组蛋白 Recombinant Corticosteroid Binding Globulin (CBG)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species    Homo sapiens (Human)
Product No.    RPB226Hu02
Source    Prokaryotic expression
Host    E.coli
Purity    > 95%
UOM    50ug
Predicted Molecular Mass    19.2kDa
Applications    SDS-PAGE; WB; ELISA; IP.
Endotoxin Level    <1.0EU per 1µg (determined by the LAL method)
Residues    Glu218~Arg378 with N-terminal His-Tag
皮质醇结合球蛋白(CBG)重组蛋白
Formulation    Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
EEN FYVDETTVVK VPMMLQSSTI SYLHDSELPC QLVQMNYVGN GTVFFILPDK GKMNTVIAAL SRDTINRWSA GLTSSQVDLY IPKVTISGVY DLGDVLEEMG IADLFTNQAN FSRITQDAQL KSSKVVHKAV LQLNEEGVDT AGSTGVTLNL TSKPIILR
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
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