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Purified anti-human Perforin

产品信息
  • 上海科敏生物经销国内外知名品Pai试剂抗体,折扣低,货期快,欢迎新老客户咨询订购!
    Purified anti-human Perforin参数介绍>>>>
    ClonedG9
    IsotypeMouse IgG2b, κ
    ReactivityHuman, Cross-Reactivity: Bovine (Cow, Cattle); does not cross-react with mouse1.
    ImmunogenPurified granules from the human lymphoma cell line
    FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
    PreparationThe antibody was purified by affinity chromatography.
    Concentration0.5mg/ml
    Recommended UsageEach lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
    ApplicationsICFC - Quality tested
    IP, IF, IHC - Reported in the literature
    Application Notes

    Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.

    Application References

    1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
    2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
    3. Watanabe N, et al. 1997. Blood 90:3662.
    4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
    5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
    6. Chen H, et al. 2005. J. Immunol. 175:591.
    7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
    8. Wood SM, et al. 2009. Blood 114:4117. PubMed

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    Description

    Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

    StorageThe antibody solution should be stored undiluted at 4°C.

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