Purified anti-human Perforin
- 品牌:Biolegend
- 型号:308102
- 产地:美国
- 供应商:上海恒斐生物科技有限公司
- 供应商报价:¥2275
- 标签:Purified anti-human Perforin、Purified anti-human Perforin价格、Purified anti-human Perforin厂家、、、上海恒斐生物科技有限公司
| Clone | dG9 |
| Isotype | Mouse IgG2b, κ |
| Reactivity | Human, Cross-Reactivity: Bovine (Cow, Cattle); does not cross-react with mouse1. |
| Immunogen | Purified granules from the human lymphoma cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | ICFC - Quality tested IP, IF, IHC - Reported in the literature |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4. |
| Application References | 1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC) |
| Description | Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. |
| Storage | The antibody solution should be stored undiluted at 4°C. |