| Clone | TR75-54.7 |
| Isotype | Armenian Hamster IgG |
| Reactivity | Mouse |
| Immunogen | E. coli -expressed mouse Type II TNFR |
| Formulation | 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test. |
| Preparation | The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
| Concentration | 1mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA and/or immunofluorescent staining with flow cytometric analysis. For ELISA detection applications, a concentration range of 0.125-0.5 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF R Type II/p75 recombinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | FC - Quality tested |
| Application Notes | Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). |
| Application References | 1. Sheehan KC, et al. 1995. J. Exp. Med. 181:607. |
| Description | CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The TR75-54.7 antibody has been shown to block ligand-induced receptor signaling. |
| Storage | The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions. |
