1)无菌室及无菌操作台(laminarflow)用紫外灯照射30-60分钟灭菌,70%ethanol擦拭无菌操作抬面,并开启无菌操作台风扇运转10分钟。2)细胞用的培养基如有相同切记不可共享培养基,以避免失误混淆或细胞间污染。 (注:实验操作应在抬面之ZY无菌区域,勿在边缘之非无菌区域操作。)培养细胞将复合细胞计数要求的细胞悬液分装入培养瓶内,将培养瓶放入37℃和5%CO2的培养箱内2-4小时(或者24-48小时)后换液继续培养培养,换液的时间由细胞情况而定。
Alexa Fluor 555标记的小鼠抗人IgG 0.1ml Mouse Anti-human IgG/Alexa Fluor 555
Cy7标记的小鼠抗大鼠IgG 0.1ml Mouse Anti-rat IgG/Cy7
Alexa Fluor 647标记的兔抗亲和素 0.1ml Rabbit Anti-Avidin/Alexa Fluor 647
Alexa Fluor 488标记的兔抗鸡IgM 0.1ml Rabbit Anti-chicken IgM/Alexa Fluor 488
辣根过氧化物酶标记的兔抗豚鼠IgG 0.1ml Rabbit Anti-Guinea pig IgG/HRP
罗丹明标记的兔抗人IgM 0.3ml Rabbit Anti-human IgM/RBITC
Alexa Fluor 350标记的兔抗猴IgM 0.1ml Rabbit Anti-MK IgM/Alexa Fluor 350
PE-Cy5.5标记的兔抗大鼠IgM 0.1ml Rabbit Anti-rat IgM/PE-Cy5.5
Alexa Fluor 555标记的驴抗人IgG 0.1ml Do
nkey Anti-human IgG/Alexa Fluor 555
β淀粉样肽1-28(多肽蛋白) 0.5mg Beta-Amyloid(1-28)
金属蛋白酶组织YZ因子-2(抗原) 0.5mg TIMP-2(Tissue Inhibitor of me
talloproteinase-2)
免疫球蛋白结合蛋白-1 0.5mg Immunoglobuin binding protein-1(CD79A)IGBP-1
G蛋白偶联受体-2(抗原) 0.5mg CKR-L2( G protein-coupled receptor-2 )
磷酸化活化复制因子2抗原 0.5mg phospho-ATF2(pSer490/pSer498)
风疹病毒糖蛋白多肽片段 0.5mg glycoprotein (E1 glycoprotein) [Rubella virus]
嗜酸粒细胞趋化蛋白14抗原 0.5mg CCL14/HCC-1(C-C motif chemokine 14)
细胞角蛋白14抗原 0.5mg CK14/17/42/10 peptide
多能发育相关基因2抗原 0.5mg DPPA2 (developmental pluripotency associated gene2) MF498 (25 mg) N-[[[4-(5,9-diethoxy-6,8-dihydro-6-oxo-7H-pyrrolo[3,4-g]quinolin-7-yl)-3-methylphenyl]methyl]sulfonyl]-2-methoxy-benzeneacetamide
L-(+)-Cystathio
nine (50 mg) S-[(2R)-2-amino-2-carboxyethyl]-L-homocysteine
N-Acetyl-4-benzoquinone imine (1 mg) N-Acetyl-p-benzoquino
nimine|NAPQI; N-(4-oxo-2,5-cyclohexadien-1-ylidene)-acetamide
Acetyl Coenzyme A (sodium salt) (25 mg) Acetyl-CoA; S-acetate coenzyme A, trisodium salt
AWD 131-138 (10 mg) Imepitoin; 1-(4-chlorophenyl)-1,5-dihydro-4-(4-morpholinyl)-2H-imidazol-2-one
TGR5 Receptor Ago
nist (10 mg) 3-(2-chlorophenyl)-N-(4-chlorophenyl)-N,5-dimethyl-4-isoxazolecarboxamide
AB-CHMINACA me
tabolite M3A (5 mg) (1-((4-hydroxycyclohexyl)methyl)-1H-indazole-3-carbonyl)-L-valine
Trimethoprim (25 g) Trimpex; 5-[(3,4,5-trimethoxyphenyl)methyl]-2,4-pyrimidinediamine
BIBR 1532 Telomerase Inhibitor X; 2-[[(2E)-3-(2-naphthalenyl)-1-oxo-2-buten-1-yl]amino]-benzoic acid
ZM 39923 (hydrochloride JAK3 Inhibitor IV; 3-[(1-methylethyl)(phenylmethyl)amino]-1-(2-naphthalenyl)-1-propanone, monohydrochloride
人胚肾上皮细胞;GC-293T价格RGFP 966 (10 mg) (2E)-N-(2-amino-4-fluorophenyl)-3-[1-(3-phenyl-2-propen-1-yl)-1H-pyrazol-4-yl]-2-propenamide
AS-605240 (potassium salt) (25 mg) 5-(6-quinoxalinylmethylene)-2,4-thiazolidinedione, mo
nopotassium salt
cis-Tramadol-d6 (hydrochloride) (5 mg) Contramal-d6|Tramal-d6|Ultram-d6|Zydol-d6; rel-2R-[(dimethylamino)methyl]-1R-(3-methoxyphenyl)-cyclohexanol-d6, monohydrochloride
JWH 015 RM (5 mg) (2-methyl-1-propyl-1H-indol-3-yl)-1-naphthalenyl-methanone
Streptavidin:SureLight APC (1 mg) Streptavidin:SureLight APC
人胚肾上皮细胞;GC-293T价格操作步骤:
1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。