重组人血小板因子4|Human PF-4|Platelet Factor-4 Human Recombinant (CXCL4)ProSpec（蛋白质专家）是以色列一家具有超过16年历史的，专业从事细菌来源重组蛋白生产的先进生物技术公司。ProSpec生产多种细胞因子、生长因子、趋化因子、激素、酶、病毒抗原和许多其他重组蛋白。在研究团队和科学家们的努力下，ProSpec已经拥有超过1700种可用于癌症、细胞凋亡、发育、内分泌学、免疫学、神经科学、蛋白酶及干细胞研究用重组蛋白、多肽和抗体。10多年蛋白纯化方面的经验使ProSpec公司生产成本低、产量高、效率高，其蛋白产品不仅纯度高、生物活性高，价格也非常具有优势。同时ProSpec针对客户的需要提供自定义重组蛋白生产服务。 ProSpec向客户提供蛋白质产品、服务和技术授权。因为创新的产品、的客户服务和对细节的注重，ProSpec在科学界建立了良好的信誉，具有广泛的客户群，包括世界各地的学术、政府研究机构，生物技术和制药企业以及医院和基准实验室。
产品详情：CATALOGUE NUMBER
CHM-350
SYNONYMS
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
INTRODUCTION
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy.
DESCRIPTION
CXCL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa.
SOURCE
Escherichia Coli.
PHYSICAL APPEARANCE
Sterile Filtered white lyophilized powder.
FORMULATION
The CXCL4 protein was lyophilized after extensive dialysis against 50mM Tris-HCl pH 8.0 and 150mM NaCl buffer.
SOLUBILITY
It is recommended to reconstitute the lyophilized CXCL4 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized CXCL4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
AMINO ACID SEQUENCE
The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu-Asp.
BIOLOGICAL ACTIVITY
The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 µg/ml.
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
温馨提示：不可用于临床ZL。
