Human PDGF-AB/重组人血小板源生长因子-AB

Human PDGF-AB/重组人血小板源生长因子-AB
产品编号：200-053S/200-053/200-054
产品规格：2μg/5μg/20μg 
Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
Source: E.coli
Molecular Weight: 25.5 kDa
Purity: >95% by SDS-PAGE and visualised by silver stain
Endotoxin level/内毒素水平: < 0.1 ng per ug of PDGF-AB
Stabilizer: none
Buffer: 50 mM acetic acid
Formulation: lyophilized
Biological Activity: The as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
Stability: The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AB is best stored at –20°C to –70°C. Avoid repeated freeze-thaw cycles.
Usage: Human PDGF-AB is offered for research use. Not for drug use. Not for human use.