Stemgent® Purified Mouse Anti-Human CD29 Antibody

Stemgent® Purified Mouse anti-Human CD29 Antibody was screened on normal human mesenchymal stem cells (MSC) by immunocytochemistry (ICC) and flow cytometry (FC) and selected as the best CD29 antibody for characterizing and/or isolating human mesenchymal stem cells. The CD29 molecule is a 130 kDa single chain type I glycoprotein (also known as integrin β1 chain subunit) that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources^1-3. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions^4-6.

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2. Campagnoli, C., Roberts, I.A., Kumar, S., Bennett, P.R., Bellantuono, I., Fisk, N.M. (2001) Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood 98: 2396-402.
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4. Cervella, P., Silengo, L., Pastore, C., Altruda, F. (1993) Human beta 1-integrin gene expression is regulated by two promoter regions. J Biol Chem 268: 5148-55.
5. Hemler, M.E., Lobb, R.R. (1995) The leukocyte beta 1 integrins. Curr Opin Hematol 2: 61-7.
6. Brakebusch, C., Fassler, R. (2005) beta 1 integrin function in vivo: adhesion, migration and more. Cancer Metastasis Rev 24: 403-11.