Stemgent® Purified Mouse anti-Human CD166 Antibody

Product Overview

 

Stemgent® Purified Mouse anti-Human CD166 Antibody was screened on normal human mesenchymal stem cells (MSC) by flow cytometry (FC) and selected as the best CD166 antibody for characterizing and/or isolating human mesenchymal stem cells. CD166, also known as ALCAM (activated leukocyte cell adhesion molecule), is a member of the immunoglobulin superfamily with five extracellular immunoglobuli -like domains. It facilitates heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. While expressed in a wide variety of tissues and cells, ALCAM is restricted to subsets of cells usually involved in dynamic growth and/or migration processes^1-2. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources^3-5.

 

References

1. van Kempen, L.C., Nelissen, J.M., Degen, W.G., Torensma, R., Weidle, U.H., Bloemers, H.P., Figdor, C.G., Swart, G.W. (2001) Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction. J Biol Chem 276: 25783-90.
2. Bowen, M.A., Patel, D.D., Li, X., Modrell, B., Malacko, A.R., Wang, W.C., Marquardt, H., Neubauer, M., Pesando, J.M., Francke, U., et al. (1995) Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med 181: 2213-20.
3. Dvorakova, J., Hruba, A., Velebny, V., Kubala, L. (2008) Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets. Cell Biol Int 32: 1116-25.
4. Lian, Q., Lye, E., Suan Yeo, K., Khia Way Tan, E., Salto-Tellez, M., Liu, T.M., Palanisamy, N., El Oakley, R.M., Lee, E.H., Lim, B., Lim, S.K. (2007) Derivation of clinically compliant MSCs from CD105+, CD24- differentiated human ESCs. Stem Cells 25: 425-36.
5. Anker, P.S., Noort, W.A., Scherjon, S.A., Kleijburg-van der Keur, C., Kruisselbrink, A.B., van Bezooijen, R.L., Beekhuizen, W., Willemze, R., Kanhai, H.H., Fibbe, W.E. (2003) Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica 88: 845-52.