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Pierce Biotinylated Protein Interaction Pull-Down Kit Pull-Down 生物素-蛋白质相互作用试剂盒

产品信息
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    Applications:
    • Discover a new protein interaction complex using a biotinylated protein as the bait to capture a putative binding partner from a cell lysate or other sample
    • Confirm expression of a known protein interaction target in a cell lysate
    • Determine the ability of two purified proteins to interact (bind) in various buffer conditions
    • Extract protein:protein interaction information from in vitro transcription/ translation lysates
    Highlights:
    • Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions
    • Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein stains)
    • Adaptable to single- or multiple-sample demands
    • Flexible pull-down format uses spin cups for easy and efficient manipulation of streptavidin agarose beads
    • Pierce Biotinylated Protein Interaction Pull-Down Kit
      Sufficient materials for 25 pull-down assays using a biotinylated protein as the bait.
      Kit contains:
      Steptavidin Agarose Beads 1.5 ml resin
      Tris Buffered Saline Pack (makes 500 ml) 1 pack
      Biotin Blocking Buffer 15 ml
      Wash Buffer (Acetate, pH 5.0) 100 ml
      Elution Buffer (pH 2.8) 50 ml
      Spin Cup Columns and Accessories 27 columns
      Collection Tubes 200 tubes
    温馨提示:不可用于临床ZL。
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