Description: This antibody is raised from rabbits with immunization of NG- mono-methyl arginine-KLH conugates and specifically isolated and affinity purified using monomethylarginine antigen. The affinity purified methyl arginie antibodys useful for detection, isolation, localization of proteins with NG-methylated arginine residues. Sepcies: Rabbit Formulation: 250 µg/mL in PBS, 50% glycerol. Immunogen: NG-mono-methyl arginine-KLH conjugates Purification: Antigen-specific affinity chromatography. Adsorbed with dimethyl arginine Specificity: Recognize proteins with methylated arginine residues. Not cross-react with acetylated proteins and mono- and dimethylated proteins Tested: Human, mouse, pig. Applications: ELISA (1:2000), Western blot(1:1000), IP(5 μg/mg protein sample), IF(5 μg/mL), IHC(5 μg/mL). Scientific Description: Arginine methylation is a very common posttranslational modification, that occurs predominantly in the nucleus and the main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation. Reference: Polevoda B and Sherman F.Mol Microbiol.2007, 65:590-606
