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Mouse Interleukin 17,IL-17 ELISA Kit使用原理
货号:FK-QZ2901
Mouse Interleukin 17,IL-17 ELISA Kit使用原理
The kit was a solid phasesandwich enzyme-linked immunosorbent assay (ELISA), and the AVPconcentrations of the standard samples and unknown concentrations ofsamples were detected by the addition of microporous enzyme plates.. Atthe same time, the AVP and the biotin labeled antibodies were incubatedat the same time.. After washing, the addition of Pro labeled HRP. Afterthe warming and washing, the removal of the non binding enzymeconjugate, and then added to the substrate a, B, and the enzymeconjugate at the same time. Produce color. The color depth and theconcentration of AVP in the sample was proportional to the concentrationof the sample..
Operation notes
2 the experimental plate should be immediately put back in the bag,seal the preservation, lest metamorphism.
4 use a disposable suction head lest cross contamination, draw thetermination liquid and substrate B, a liquid, to avoid using the metalpart of the sample.
6 wash the enzyme plate should be fully dry, do not absorb water paperdirectly into the enzyme labeled pores in the water absorption.
8 add reagents should be consistent in order, to ensure that allreaction plate hole incubation time.
1 the correlation coefficient r value of linear regression and theexpected concentration of sample was 0.92 above.
Detection range:
Save condition and its validity:
2 validity: 6 months
