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重组鸡瘦素受体/Leptin Receptor Chicken

产品信息
重组鸡瘦素受体/Leptin Receptor Chicken
产品编号：CYT-509 
产品规格：5μg/20μg/1mg
Catalogue NumberCYT-509
SynonymsOB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.
DescriptionLeptin Binding Domain Chicken Recombinant also called Leptin Receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Chicken Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 420-626 of chicken leptin receptor.

The Leptin Binding Domain is purified by proprietary chromatographic techniques.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution at a concentration of 0.4 mg/ml.
FormulationThe protein was filter sterilized and stored at 4°C (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl.
StabilitySterile solutions at 0.5mg/ml or less are stable at 4°C for several months.
PurityGreater than 99.0% as determined by:

(a) Analysis by SEC-HPLC.

(b) Analysis by SDS-PAGE.
Amino acid sequenceThe sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile Biological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Protein contentProtein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 



2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
UsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.



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