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重组结核杆菌热休克蛋白-65/HSP65 Mycobacteriu

产品信息
重组结核杆菌热休克蛋白-65/HSP65 Mycobacterium
产品编号：HSP-065 
产品规格：2μg/10μg/100μg
Catalogue NumberHSP-065
SynonymsProtein Cpn60-2, groEL protein-2, 65 kDa antigen, Heat shock protein 65, Cell wall protein A, Antigen A, groL2, groEL-2.
IntroductionHeat shock proteins induce pro-inflammatory cytokines. Mycobacterial HSPs participate in cytokine expression resulting from infection by M. tuberculosis. Furthermore, HSPs stabilize cellular proteins in response to various sources of stress or injury. HSP65 is one of the most essential defending immunogens against the tuberculosis infection. HSP65 is presented to human CD41 T cells in association with multiple HLA-DR molecules. The M. tuberculosis HSP65 signals through TLR4.
DescriptionRecombinant Mycobacterium Tuberculosis HSP65 is produced in E.coli and has a Mw of 57.4 kDa. The HSP65 protein is fused to His-Tag at N-Terminus and purified by stabdard chromatography techniques.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe HSP65 protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na-phosphate pH-7.4, 130mM NaCl and 2.5mM KCl.
SolubilityIt is recommended to reconstitute the lyophilized HSP-65 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized HSP65 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSP65 should be stored at 4°C between 2-7 days and for future use below 

-18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.
Amino Acid SequenceHHHHHHGSAKTIAYDEEARRGLERGLNALADAVKV

TLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEDPY

EKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVRE

GLRNVAAGANPLGLKRGIEAVEKVTETLLKGAKEVET

KEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITV

EESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAV

LEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIEDVEGE

ALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAIL

TGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVE

GAGDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKL

AGGVAVKAGAATEVELKERKHRIEDAVRNAKAAVEEGIV

AGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPL

KQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLA

AGVADPVKVTRSALQNASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF.
UsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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