Human endogenous soluble VEGFR-1/Flt-1 (esFlt-1)/重组人内源可溶性血管内皮生长因子-1/Flt-1 (esFlt-1)

Human endogenous soluble VEGFR-1/Flt-1 (esFlt-1)/重组人内源可溶性血管内皮生长因子-1/Flt-1 (esFlt-1)
产品编号：S01-009/S01-010
产品规格：5μg/20μg
Description: Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR-1) is the naturally occurring form and was cloned from total RNA of human umbilical vein endothelial cells. The recombinant mature sVEGFR-1 is a glycosylated monomeric protein with a mass of approximately 96 kDa. The soluble receptor precursor protein consists of the first 6 extracellular domains (Met1-His688) containing the unique 31 amino acids residues at the C-terminus.
Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly, a naturally occurring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
Source: Insect cells
Molecular Weight: 96 kDa
Subunit: glycosylated monomer
Purity: > 85%, by SDS-PAGE and visualized by silver stain
Endotoxin level/内毒素水平: < 0.1 ng per μg of sVEGFR-1
Stabilizer: none
Buffer: PBS
Formulation: lyophilized
Biological Activity: The activity of sVEGFR-1 was determined by its ability to abolish the binding of iodinated VEGF to solid surfaces or cell surface receptors. The for this effect is typically 10 ng/ml
Reconstitution: The lyophilized sVEGFR-1 is soluble in water and most aqueous buffers. The lyophilized sVEGFR-1 should be reconstituted in PBS to a concentration not lower than 100 ng/ml.
Stability: Lyophilized samples are stable for greater than six months at –70°C. Reconstituted sVEGFR-1 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Usage: sVEGFR-1 is offered for research use. Not for drug use. Not for human use!