Murine NOGGIN/重组小鼠Noggin蛋白

Murine NOGGIN/重组小鼠Noggin蛋白
产品编号：M10-124
产品规格：20μg
Description: Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.
Source: E. coli
Molecular Weight: 46.4 kDa
Purity: > 95% by SDS-PAGE
Endotoxin level/内毒素水平: < 0.1 ng per μg of NOGGIN
Stabilizer: none
Formulation: lyophilized
Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected for this effect is 1.0-2.0 ng/ml of Noggin.
Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted NOGGIN is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C.
Avoid repeated freeze-thaw cycles.
Usage: Murine NOGGIN offered for research use. Not for drug use. Not for human use.