产品库
登录
首页 找仪器 社区 百科 新品 供应商 品牌 应用 资料 仪企号 展会 标准 求购 招中标

Ang K1-3 Human/重组人血管抑素纤维蛋白溶酶原

产品信息
Ang K1-3 Human/重组人血管抑素纤维蛋白溶酶原
产品编号：PRO-284
产品规格：20μg/100μg/1mg
Synonyms Angiostatin, Angiostatin Kringles 1-3, Ang K1-3.
Introduction Ang K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.
Description Angiostatin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 259 amino acids and having a molecular mass of approximately 30.0 kDa.

The Ang K1-3 is purified by proprietary chromatographic techniques.
Source Escherichia coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM NaAc, pH5.5, 4% mannitol.
Solubility We recommend to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Stability The lyophilized Angiostatin K1-3 is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C.

Avoid repeated freeze/thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.
Amino Acid Sequence VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLKGTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP.
Biological Activity The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 0.55×105 Units/mg.
Protein content Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of Ang K1-3 as a Reference Standard.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
信息声明：本产品供应信息由仪器网为您整合，供应商为（厦门慧嘉生物科技有限公司），内容包括 （ Ang K1-3 Human/重组人血管抑素纤维蛋白溶酶原）的品牌、型号、技术参数、详细介绍等；如果您想了解更多关于 （ Ang K1-3 Human/重组人血管抑素纤维蛋白溶酶原）的信息，请直接联系供应商，给供应商留言！
供应商产品推荐
    您可能感兴趣的产品