ab179884 Leptin Human SimpleStep ELISA Kit

Product nameLeptin Human SimpleStep ELISA? Kit See all Leptin kitsDetection methodColorimetricPrecision Intra-assay Sample n Mean SD CV% HeLa extract 5 2.4% Sample typeCell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate PlasmaAssay typeSandwich (quantitative)Sensitivity 5.3 pg/mlRange11.7 pg/ml - 750 pg/mlRecovery Sample specific recovery Sample type Average % Range Serum 95 90% - 99% Cell culture media 116 114% - 119% Heparin Plasma 116 108% - 122% EDTA Plasma 106 103% - 108% Citrate Plasma 109 104% - 111% Assay time1h 30m Assay durationOne step assaySpecies reactivityReacts with: Human Product overview Abcam’s Leptin in vitro SimpleStep ELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Leptin protein in Human serum, plasma and cell culture supernatants. The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. Notes Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause in obesity.Acute low levels of leptin triggers increased appetite and chronic leptin deficiency can block the onset of puberty. Tested applicationsSandwich ELISAmore details PlatformMicroplate Properties Storage instructionsStore at +4°C. Please refer to protocols. Components 1 x 96 tests 10X Leptin Capture Antibody 1 x 600μl 10X Leptin Detector Antibody 1 x 600μl 10X Wash Buffer PT 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Leptin Human Lyophilized Recombinant 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml Research Areas Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Atherosclerotic proteins ELISA kits Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Growth factors and hormones ELISA kits FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.Sequence similaritiesBelongs to the leptin family.Cellular localizationSecreted. Information by UniProt Alternative names FLJ94114 LEP LEP_HUMAN see allDatabase links Entrez Gene: 3952 Human Omim: 164160 Human SwissProt: P41159 Human Unigene: 194236 Human