ab174445 ICAM1 （CD54） Human SimpleStep ELISA...

Product nameICAM1 (CD54) Human SimpleStep ELISA? Kit See all ICAM1 kitsDetection methodColorimetricPrecision Intra-assay Sample n Mean SD CV% Overall 6 6% Inter-assay Sample n Mean SD CV% Overall 24 7% Sample typeCell culture supernatant, Serum, Plasma, Cell culture extracts, Adherent cells, Tissue Homogenate, Tissue LysateAssay typeSandwich (quantitative)Sensitivity 1.6 pg/mlRange39.06 pg/ml - 2500 pg/mlRecovery Sample specific recovery Sample type Average % Range Serum 103 99% - 110% Plasma 113 96% - 126% Cell culture media 98 91% - 102% Extraction Buffer 87 82% - 97% Assay time1h 30m Assay durationOne step assaySpecies reactivityReacts with: Human Does not react with MouseProduct overview Abcam’s ICAM1 (CD54) in vitro SimpleStepELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM-1 protein in Human cell culture supernatant, plasma, serum and cell lysate samples. The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. Notes ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system. The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail. ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes. Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly. ICAM-1 expression increases in the vascular endothelium, macrophages and lymphocytes in response to cytokine stimulation (IL-1 and TNF). Furthermore, ligation of ICAM-1 up-regulates its own expression in a positive-feedback loop to maintain a pro-inflammatory environment conducive to leukocytes endothelial transmigration. Soluble ICAM-1 is reported to be present in normal serum, cerebrospinal fluid, urine and bronchoalveolar lavage fluid. Elevated levels of soluble ICAM-1 have been associated with multiple organ failure, atherosclerosis, autoimmune disorders, diabetes, obesity, hypertension, liver disease as well as cancer, and several studies have correlated serum levels of sICAM-1 with the severity of these diseases. Tested applicationsSandwich ELISAmore details PlatformMicroplate