G418 disulfate/G418硫酸盐
产品编号：G001
产品规格：1g/5g/25g/100g
| CAS Number | 108321-42-2 |
|---|
| Molecular Formula | C20H40N4O10 · 2 H2SO4 |
|---|
| Molecular Weight | 692.71 g/mol |
|---|
| Form | Powder |
|---|
| Purity Level | ≥90.0% |
|---|
| Solubility | Water: Soluble |
|---|
| Source | Micromonospora Rhodorangea |
|---|
| Ammonia | <0.1% |
|---|
| Water Content (Karl Fisher) | <12.0% |
|---|
| Boiling Point | 1012.1 °C |
|---|
| Flash Point | 565.9 °C |
|---|
| Optical Rotation | +104o to +121o |
|---|
| Storage Conditions | 2-8 °C |
|---|
Description
G418 disulfate also known as geneticin, is an aminoglycoside antibiotic commonly used in plant and mammalian cell culture as a selectable marker.
TOKU-E offers three forms of G418: G418 disulfate (G001), G418 disulfate, EvoPure (G030), and G418 disulfate solution (G020). G418 disulfate and G418 disulfate, EvoPure? are soluble in aqueous solution. G418 disulfate solution is G418 dissolved in water at 50 mg/mL. EvoPure? products are purified single antibiotic fractions, most >99% pure.
Mechanism
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
Plant Biology Applications
Gene selection (see below). Dong and McHughen (1992) compared the use of kanamycin and geneticin as a selection agent in the genetic transformation of flax by Agrobacterium mediated transformation. Results showed G418 to be the more potent selective agent. Experiments in sweet potato showed G418 to be a more reliable selection agent with a higher transformation efficiency compared to kanamycin.
Mammalian Biology Applications
