Actinomycin D/放线菌素D
产品编号：A001
产品规格：25mg
| CAS Number | 50-76-0 |
|---|
| Molecular Formula | C62H86N12O16 |
|---|
| Molecular Weight | 1255.42 g/mol |
|---|
| Form | Powder |
|---|
| Appearance | Red or orange powder |
|---|
| Purity Level | ≥98.0% |
|---|
| Solubility | Acetone: 10 mg/mL
Acetonitrile: 10 mg/mL
Water: 0.5 mg/mL |
|---|
| Source | Streptomyces |
|---|
| Melting Point | 243-248 °C |
|---|
| Storage Conditions | -20 °C |
|---|
Description
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis against tumor cells.
Mechanism
Actinomycin is a cyclic polypeptide-containing antibiotic that binds to DNA and inhibits RNA polymerase activity. Because Actinomycin can bind DNA duplexes, it can also interfere with DNA replication, although other chemicals such as hydroxyurea are better suited for use in the laboratory as inhibitors of DNA synthesis. Actinomycin D and its fluorescent derivative,7-aminoactinomycin D (7-AAD), are used as stains in microscopy and flow cytometry applications. The affinity of these stains/compounds for GC-rich regions of DNA strands makes them excellent markers for DNA. 7-AAD binds to single stranded DNA; therefore it is a useful tool in determining apoptosis and distinguishing between dead and alive cells.
Plant Biology Applications
Actinomycin D is used in plant tissue culture to inhibit plant growth through tissue deformation. It also acts as an RNA-synthesis inhibitor. Exposure of Actinomycin D at concentrations of 12-20 ug/ml for 3-4 hours in pea and soybean plants caused significant tissue deformation.
Cancer Applications
