Asymmetric DiMethyl-Histone H3-R2 pAb
- 品牌：Bio-Swamp
- 型号：PAB33585
- 供应商：武汉华联科生物技术有限公司
- 供应商报价：¥440 - 2480
- 标签：Asymmetric DiMethyl-Histone H3-R2 pAb、Asymmetric DiMethyl-Histone H3-R2 pAb价格、Asymmetric DiMethyl-Histone H3-R2 pAb厂家、、Bio-Swamp、武汉华联科生物技术有限公司
产品库
| Product name | Asymmetric DiMethyl-Histone H3-R2 pAb |
| Isotype | IgG |
| Form | Lyophilized or Liquid |
| Tested applications | WB,IHC,IF,IP,ChIP,ChIPseq |
| Species reactivity | Human,Mouse,Rat,Other (Wide Range) |
| Host Species | Rabbit |
| Purity | Affinity purification |
Immunogen Information：
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3 |
| Calculated molecular weight | 15kDa |
| Observed molecular weight | 17kDa |
| Swiss Prot | Q16695 |
| Gene ID (NCBI) | 8290 |
| Alternative Names | H3.4;H3/g;H3FT;H3t |
Application Notes：
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| WB | 1:500 - 1:2000 |
| IHC | 1:50 - 1:200 |
| IF | 1:50 - 1:200 |
| IP | 1:50 - 1:200 |
| ChIP | 1:20 - 1:100 |
| CHIPseq | 1:20 - 1:100 |
| Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody. | Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody. |
| Immunohistochemistry of paraffin-embedded rat brain using DiMethyl-Histone H3-R2 antibody. | Immunohistochemistry of paraffin-embedded human colon using DiMethyl-Histone H3-R2 antibody. | Immunohistochemistry of paraffin-embedded mouse brain using DiMethyl-Histone H3-R2 antibody. |
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R2 antibody.
Background：
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Storage：
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
