| Product name | Asymmetric DiMethyl-Histone H3-R17 pAb |
| Isotype | IgG |
| Form | Lyophilized or Liquid |
| Tested applications | WB,IHC,IF,IP |
| Species reactivity | Human,Mouse,Rat,Other (Wide Range) |
| Host Species | Rabbit |
| Purity | Affinity purification |
Immunogen Information：
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3 |
| Calculated molecular weight | 15kDa |
| Observed molecular weight | 17kDa |
| Swiss Prot | P68431 |
| Gene ID (NCBI) | 8356 |
| Alternative Names | H3/j;H3FJ |
Application Notes：
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| WB | 1:500 - 1:2000 |
| IHC | 1:50 - 1:200 |
| IP | 1:50 - 1:200 |
| FC | 1:50 - 1:200 |
| Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody. | Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody. |
| Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody. | Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H3-R17 antibody. | Immunohistochemistry of paraffin-embedded human kidney cancer using Asymmetric DiMethyl-Histone H3-R17 antibody. |
| Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody. | Immunohistochemistry of paraffin-embedded rat testis using Asymmetric DiMethyl-Histone H3-R17 antibody. | Immunohistochemistry of paraffin-embedded human placenta using Asymmetric DiMethyl-Histone H3-R17 antibody. |
| Immunohistochemistry of paraffin-embedded mouse brain using Asymmetric DiMethyl-Histone H3-R17 antibody. | Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody. |
Background：
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Storage：
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
