Human Protein patched homolog 2, PTCH2 ELISA KIT

Human Protein patched homolog 2, PTCH2 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

PTCH2,PTC2; patched homolog 2; patched 2, Protein patched homolog 2

 

Search name

Human PTCH2 ELISA KIT ,Human PTC2 ELISA KIT ,Human patched homolog 2 ELISA KIT ,Human patched 2 ELISA KIT ,Human  Protein patched homolog 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human PTC2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toPTC2, During the reaction,PTC2in the sample or standard competes with a fixed amount of biotin-labeledPTC2 for sites on a pre-coated Monoclonal antibody specific to PTC2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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