Human Carbamoyl- phosphate synthase [ammonia], mitochondrial, CP

Human Carbamoyl- phosphate synthase [ammonia], mitochondrial, CPS1 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Carbamoyl-phosphate synthase [ammonia], mitochondrial),Carbamoyl-phosphate synthetase I,CPSase I,CPS1,CPSM,CPSASE1; PHN; carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoylphosphate synthetase I; carbamoyl-phosphate synthase 1 mitochondrial

 

Search name

Human Carbamoyl-phosphate synthase [ammonia] mitochondrial) ELISA KIT ,Human Carbamoyl-phosphate synthetase I ELISA KIT ,Human CPSase I ELISA KIT ,Human CPS1 ELISA KIT ,Human CPSM ELISA KIT ,Human CPSASE1 ELISA KIT ,Human PHN ELISA KIT ,Human carbamoylphosphate synthetase I ELISA KIT ,Human carbamoyl-phosphate synthase 1 mitochondrial ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human carbamoyl-phosphate synthase [ammonia], mitochondrial,CPSM concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPSM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CPSM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CPSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPSM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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