Product Name:Human GPI ethanolamine phosphate transferase 1, PIGN ELISA KIT
Packing:96T
Catalog No.:ELI-22004h
Gene Name:PIGN
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:GPI ethanolamine phosphate transferase 1(PIGN
Alternative Name:PIGN,GPI ethanolamine phosphate transferase 1(PIGN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human GPI ethanolamine phosphate transferase 1, PIGN ELISA KIT allows for the in vitro quantitative determination of GPI ethanolamine phosphate transferase 1(PIGN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human GPI ethanolamine phosphate transferase 1, PIGN ELISA KIT has been pre-coated with an GPI ethanolamine phosphate transferase 1(PIGN antibody specific to GPI ethanolamine phosphate transferase 1(PIGN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GPI ethanolamine phosphate transferase 1(PIGN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GPI ethanolamine phosphate transferase 1(PIGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GPI ethanolamine phosphate transferase 1(PIGN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
