Mouse GPI ethanolamine phosphate transferase 3, Pigo ELISA KIT Product Name:Mouse GPI ethanolamine phosphate transferase 3, Pigo ELISA KIT
Packing:96T
Catalog No.:ELI-35712m
Gene Name:Mouse Pigo
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Pigo
Alternative Name:Mouse GPI ethanolamine phosphate transferase 3, Pigo
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse GPI ethanolamine phosphate transferase 3, Pigo ELISA KIT allows for the in vitro quantitative determination of Mouse Pigo concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse GPI ethanolamine phosphate transferase 3, Pigo ELISA KIT has been pre-coated with an Mouse GPI ethanolamine phosphate transferase 3, Pigo antibody specific to Mouse Pigo .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pigo and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pigo , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pigo in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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