Product Name:Human Protein patched homolog 1, PTCH1 ELISA KIT
Packing:96T
Catalog No.:ELI-14033h
Gene Name:PTCH1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Protein patched homolog 1(PTCH1
Alternative Name:PTCH1,Protein patched homolog 1(PTCH1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein patched homolog 1, PTCH1 ELISA KIT allows for the in vitro quantitative determination of Protein patched homolog 1(PTCH1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein patched homolog 1, PTCH1 ELISA KIT has been pre-coated with an Protein patched homolog 1(PTCH1 antibody specific to Protein patched homolog 1(PTCH1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein patched homolog 1(PTCH1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein patched homolog 1(PTCH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein patched homolog 1(PTCH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
