Product Name:Human Monocyte differentiation antigen CD14, CD14 ELISA KIT
Packing:96T
Catalog No.:ELI-02380h
Gene Name:CD14
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Monocyte differentiation antigen CD14(CD14
Alternative Name:CD14,Monocyte differentiation antigen CD14(CD14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Monocyte differentiation antigen CD14, CD14 ELISA KIT allows for the in vitro quantitative determination of Monocyte differentiation antigen CD14(CD14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Monocyte differentiation antigen CD14, CD14 ELISA KIT has been pre-coated with an Monocyte differentiation antigen CD14(CD14 antibody specific to Monocyte differentiation antigen CD14(CD14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Monocyte differentiation antigen CD14(CD14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Monocyte differentiation antigen CD14(CD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Monocyte differentiation antigen CD14(CD14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
