Product Name:Human nicotinic acetylcholine Transferase receptor ELISA Kit
Packing:96T
Catalog No.:ELA-E1926h
Gene Name:N-AChTR
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:nicotinic acetylcholine Transferase receptor
Alternative Name:N-AChTR,nicotinic acetylcholine Transferase receptor
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human nicotinic acetylcholine Transferase receptor ELISA Kit allows for the in vitro quantitative determination of nicotinic acetylcholine Transferase receptor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human nicotinic acetylcholine Transferase receptor ELISA Kit has been pre-coated with an nicotinic acetylcholine Transferase receptor antibody specific to nicotinic acetylcholine Transferase receptor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for nicotinic acetylcholine Transferase receptor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain nicotinic acetylcholine Transferase receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of nicotinic acetylcholine Transferase receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
