Mouse Myo Inositol Oxygenase, MIOX ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
nositol oxygenase,Renal-specific oxidoreductase,Myo-inositol oxygenase,MI oxygenase,Aldehyde reductase-like 6,Kidney-specific protein 32, MIOX,myo inositol oxygenase,ALDRL6; MI oxygenase; aldehyde reductase (aldose reductase) like 6; aldehyde reductase-like 6; inositol oxygenase; kidney-specific protein 32; renal-specific oxidoreductase; myo-inositol oxygenase,KSP32,RSOR
Search name
Mouse nositol oxygenase ELISA KIT ,Mouse Renal-specific oxidoreductase ELISA KIT ,Mouse Myo-inositol oxygenase ELISA KIT ,Mouse MI oxygenase ELISA KIT ,Mouse Aldehyde reductase-like 6 ELISA KIT ,Mouse Kidney-specific protein 32 ELISA KIT ,Mouse MIOX ELISA KIT ,Mouse myo inositol oxygenase ELISA KIT ,Mouse ALDRL6 ELISA KIT , Mouse KSP32 ELISA KIT, Mouse RSOR ELISA KIT,Mouse MI oxygenase ELISA KIT ,Mouse aldehyde reductase (aldose reductase) like 6 ELISA KIT ,Mouse aldehyde reductase-like 6 ELISA KIT ,Mouse inositol oxygenase ELISA KIT ,Mouse kidney-specific protein 32 ELISA KIT ,Mouse renal-specific oxidoreductase ELISA KIT ,Mouse myo-inositol oxygenase ELISA KIT,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Inositol oxygenase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol oxygenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Inositol oxygenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inositol oxygenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inositol oxygenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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