Laminin subunit alpha 5, Lama5 ELISA KIT/ Mouse Laminin subunit alpha 5, Lama5 ELISA试剂盒

Mouse Laminin subunit alpha 5, LAMA5 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Laminin subunit alpha-5,Laminin-15 subunit alpha,Laminin-10 subunit alpha,Laminin-11 subunit alpha, LAMA5,laminin alpha-5 chain; laminin subunit alpha-5; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha; laminin alpha 5

 

Search name

Mouse Laminin subunit alpha-5 ELISA KIT ,Mouse Laminin-15 subunit alpha ELISA KIT ,Mouse Laminin-10 subunit alpha ELISA KIT ,Mouse Laminin-11 subunit alpha ELISA KIT ,Mouse  LAMA5 ELISA KIT ,Mouse laminin alpha-5 chain ELISA KIT ,Mouse laminin subunit alpha-5 ELISA KIT ,Mouse laminin-10 subunit alpha ELISA KIT ,Mouse laminin-11 subunit alpha ELISA KIT ,Mouse laminin-15 subunit alpha ELISA KIT ,Mouse laminin alpha 5 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Laminin subunit alpha-5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin subunit alpha-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Laminin subunit alpha-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Laminin subunit alpha-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Laminin subunit alpha-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF manual download

 

 

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