pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_TBfp Catalog No. PVT11018
Packing 2ug
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_TBfp Information
Function Mammal plasmids
Prokaryotic resistance: ampicillin Amp
Screening marker: red fluorescent protein mCherry, blue fluorescent protein TagBFP
Cloned strain: Escherichia coli Stbl3
Culture conditions: 37 degrees centigrade
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_TBfp Description
PHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_Tbfp is a mammalian antibody plasmid.
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_TBfp Sequence
LOCUS Exported 12090 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..12090
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 61..294
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter 1126..1230
/gene="bla"
/label=AmpR promoter
CDS 1231..2091
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2262..2850
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 3096..3425
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3276..3411
/label=SV40 ori
/note="SV40 origin of replication"
intron 4559..4624
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4754..4774
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5199..5333
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
LTR 5502..6135
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 6182..6307
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6799..7032
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 7544..7661
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS 7992..8837
/codon_start=1
/label=human FL TRAIL
/translation="MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQM
QDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQ
EKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSF
LSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILL
MKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG
"
misc_feature 8856..9429
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 9448..10158
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
promoter 10243..10742
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 10763..11464
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
misc_feature 11500..12088
/label=WPRE
温馨提示：不可用于临床ZL。
