Catalog No. PVT10835
Packing 2ug
pCMV-Gluc-1 Information
Promoter: CMV
Replicon: pUC ori, F1 ori
Terminator: SV40 poly (A) signal
Plasmid Classification: Mammalian Cells, Fluorescent Protein Reporting Vectors
Plasmid size: 4693bp
Prokaryotic resistance: Kan
Selection marker: Neo
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, transient expression
5'Sequencing Primers: CMV-F: CGCAAATGGGGGGGTAGGGGGGTG
3'Sequencing Primers: Designing Primers Based on Sequences
pCMV-Gluc-1 Multiple cloning sites
pCMV-Gluc-1 Sequence
LOCUS Exported 4693 bp ds-DNA circular SYN 29-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4693)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 29, 2016 from SnapGene Viewer 3.2.1
FEATURES Location/Qualifiers
source 1..4693
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/note="MCS"
/note="multiple cloning site"
CDS 679..1359
/codon_start=1
/product="monomeric variant of green-to-red photoswitchable
fluorescent protein EosFP (McKinney et al., 2009)"
/note="mEos2"
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
polyA_signal 1481..1602
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1609..2064)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2091..2195
/gene="bla"
/note="AmpR promoter"
promoter 2197..2554
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2405..2540
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2589..3383
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3615..3662
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3991..4579
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg
721 gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt
781 gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt
841 gatatcctga ccactgcatt catgtacggc aacagggtat tcgccaaata tccagacaac
901 atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact
961 ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc
1021 tataataaag ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag
1081 aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg
1141 ggtgatattc atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga
1201 actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac
1261 tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct
1321 gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatcat
1381 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
1441 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
1501 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
1561 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
1621 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
1681 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
1741 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
1801 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
1861 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
1921 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
