pCAGGS-Myc
PVT10729 2ug
pCAGGS-Myc Information
Promoter: CAG promoter
Replicon: Ori, SV40 ori
Terminator: beta -globin poly (A) signal
Plasmid classification: lactation cell plasmid; lactation expression plasmid; pCAG series plasmid.
Plasmid size: 4809bp
Plasmid tagging: N-Myc
Prokaryotic resistance: ampicillin Amp
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 C, aerobic, LB
Expression host: mammalian cells such as 293T
pCAGGS-Myc Descrption
pCAGGS-Myc is a mammalian eukaryotic expression plasmid. The CAG promoter drives the fusion expression of Myc tags and target genes.
pCAGGS-Myc Sequence
LOCUS Exported 4809 bp ds-DNA circular SYN 17-AUG-2017
DEFINITION synthetic circular DNA
KEYWORDS pCAGGS-Myc.dna
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4809)
FEATURES Location/Qualifiers
source 1..4809
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 1..10
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 10..39
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
polyA_site 169..224
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
promoter 757..952
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 803..938
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal 958..1092
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1330..1918)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2089..2949)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2950..3054)
/gene="bla"
/label=AmpR promoter
enhancer 3085..3464
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3466..3743
/label=chicken beta-actin promoter
intron 3744..4761
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
ORIGIN
1 gccaccatgg aacaaaaact catctcagaa gaggatctgg aattcgagct catcgatggt
61 acccgggcat gcctcgagct agcagatctt aattaattaa ggatcttttt ccctctgcca
121 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
181 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
241 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
301 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
361 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
421 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
481 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
541 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
661 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
721 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
1021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
1081 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
1141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
1201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc
1261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
1321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
1741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
1981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
2041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
2101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
2161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
2221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
2281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
2341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
2401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
2461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
2521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
2581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
2641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
温馨提示：不可用于临床ZL。
