PVT0701 2ug
Search name
pBAD/Myc-HisA,Plasmid pBAD/Myc-HisA,pBAD/Myc-HisA vector
pBAD/ Myc-HisA Description
Pbad/his and Pbad/myc-his carrier is derived from pBR322 carrier. The carrier is designed to regulate, dose-dependent expression and purify recombinant protein in Escherichia coli. The level of soluble expression of recombinant Escherichia coli protein was enhanced with E. coli Arabad promoter (PBAD). The regulatory protein Arac on the pbad/his and pbad/myc his vectors can regulate the PBAD promoter.
pBAD/ Myc-HisA Multiple cloning site
pBAD/ Myc-HisA Sequence
LOCUS Exported 4094 bp ds-DNA circular SYN 11-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS pBAD Myc-HisA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4094)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4094
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 377..406
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 422..439
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 665..751
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 843..870
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 889..980
/gene="bla"
/note="AmpR promoter"
CDS 981..1841
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2012..2600
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2786..2926
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(3190..4068)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa
361 ttcgaagctt gggcccgaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga
421 ccatcatcat catcatcatt gagtttaaac ggtctccagc ttggctgttt tggcggatga
481 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
541 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
601 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
661 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
721 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
781 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
841 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
1021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
1141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
1201 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
1261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
1321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
1381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
1441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
1501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
1561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
1621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
1681 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
1741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
2041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
