ApexPrep DNA Plasmid Miniprep Kit
- 品牌：APExBIO
- 型号：50///300
- 供应商：上海伟寰生物科技有限公司
- 供应商报价：￥400 - 2000
- 标签：ApexPrep DNA Plasmid Miniprep Kit、ApexPrep DNA Plasmid Miniprep Kit价格、ApexPrep DNA Plasmid Miniprep Kit厂家、、APExBIO、上海伟寰生物科技有限公司
产品库
| 用于分离质粒DNA |
| 规格 | 单价 | ||
|---|---|---|---|
| 50 | ￥400.00 | ||
| 300 | ￥2,000.00 |
For purification of up to 20 μg molecular biology grade plasmid DNA
The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
DNA Miniprep Protocol Using a Microcentrifuge
1. Inoculate 1-5 ml LB medium (selective antibiotic added) with a single colony, incubate with vigorous shaking at 37 °C overnight.
2. Harvest bacteria by centrifuging at > 6000 rpm for 5 min. Remove supernatant and keep pellet.
3. Resuspend bacterial pellet with 250 μl Buffer P1 and transfer to a fresh microcentrifuge tube.
4. Add 250 μl Buffer P2 and mix gently by inverting 5-6 times at room temperature.
5. Add 350 μl Buffer P3 and mix gently by inverting 5-6 times. Spin down at 12000 rpm for 10 min to remove bacterial/protein debris. Transfer supernatant to a spin column.
6. Centrifugation at 6000 rpm for 1 min. Discard flow-through.
7. (Optional), wash spin column with 0.5 ml Buffer PB; centrifuge at 6000 rpm for 1 min, and discard flow-through.
8. Wash spin column with 0.75 ml Buffer PE; centrifuge at 6000 rpm for 1 min, and discard flow-through.
9. Centrifuge at 12000 rpm for an additional 1 min to remove residual wash buffer.
10. To elute DNA, place the column in a clean 1.5 ml collection tube. Add 50 μl Buffer EB to the center of the membrane, let stand for 1 min, and centrifuge at 12000 rpm for 1 min to collect eluted DNA.
